The Proteomics Research Platform develops and applies advanced proteomics methods to further our understanding of cardiac disease, pathways, targets and drug effects.
The platform provides infrastructure for the identification and quantification of proteins and their post-translational modifications (PTMs). This includes expertise in tissue, cell, and secreted extraction, peptide separations, PTM enrichment strategies, multiplex labelling strategies, and state-of-the-art sensitive mass spectrometry (MS) for discovery-driven experiments. Our expertise extends to biofluid and blood-based proteomic profiling, tissue analyses (including low sample amount), and cell-secreted factors including extracellular vesicles.
The platform offers advice in experimental design and accurate data interpretation, and continuously develops and adapts new sample preparation techniques and MS approaches to provide multitude of solutions for individual research projects.
For more information, contact:
David Greening
Lab Head, Molecular Proteomics
Head of the Proteomics Research Platform
E: david.greening@baker.edu.au
The Proteomics Research Platform is supported by Jonathon Cross and Haoyun Fang.
Our expertise
Nano and macro proteomics of cells, tissues (fixed/frozen), clinical biopsy (formalin-fixed paraffin-embedded (FFPE), DMSO fixed, fresh frozen, or cores).- Plasma proteomic profiling (in depth, comprehensive analysis using data-independent acquisition).
- Subcellular organelle proteomics (incl. secretome and extracellular vesicles — exosomes).
- Whole proteome or immunoprecipitation/affinity purification.
- Label-free, TMT- and SILAC-based global protein quantification.
- Post-translational modification analysis (phosphorylation) (localisation and characterisation).
- Ultrahigh-pressure liquid chromatography.
- High-resolution Orbitrap mass spectrometry.
- Protein chemistry and labelling tools.
- Database searching, bioinformatics and statistical analysis.
- Comparison of proteomes.
- Expertise and applications of a wide range of cardiac, cancer, metabolic, and infectious diseases.
- Model and non-model organism expertise.
Location
Baker Heart and Diabetes Institute
75 Commercial Road, Melbourne
Victoria 3004 Australia